|Year : 2015 | Volume
| Issue : 8 | Page : 112-117
|Comparing the efficacy of xylitol-containing and conventional chewing gums in reducing salivary counts of Streptococcus mutans: An in vivo study
Rosa Haghgoo, Elahe Afshari, Tahere Ghanaat, Samaneh Aghazadeh
Department of Pediatric Dentistry, Shahed University, Tehran, Iran
|Date of Web Publication||31-Dec-2015|
Department of Pediatric Dentistry, Shahed University, Italia St., Vesal St., Keshavarz Boulevard, Tehran
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Objective: Dental caries is among the most common chronic diseases in humans. Streptococcus mutans is generally responsible for most cases of dental caries. The present study sought to compare the effects of xylitol-containing and conventional chewing gums on salivary levels of S. mutans. Materials and Methods: This study adopted a crossover design. Two type of chewing gums (one containing 70% xylitol and approved by the Iranian Dental Association, and another containing sucrose) were purchased. The participants were 32 individuals aged 18–35 years whose oral hygiene was categorized as moderate or poor based on a caries risk assessment table. Salivary levels of S. mutans were measured at baseline, after the first and second phases of chewing gums, and after the washout period. The measurements were performed on blood agar and mitis salivarius-bacitracin agar (MSBA). Pairwise comparisons were then used to analyze the collected data. Results: Salivary levels of S. mutans in both groups were significantly higher during the two stages of chewing gum than in the washout period or baseline. Moreover, comparisons between the two types of gums suggested that chewing xylitol-containing gums led to greater reductions in S. mutans counts. This effect was more apparent in subjects with poor oral hygiene than in those with moderate oral hygiene. Conclusions: Xylitol-containing chewing gums are more effective than conventional gums in reducing salivary levels of S. mutans in individuals with poor–moderate oral hygiene.
Keywords: Chewing gum, dental caries, oral hygiene, Streptococcus mutans, sucrose, xylitol
|How to cite this article:|
Haghgoo R, Afshari E, Ghanaat T, Aghazadeh S. Comparing the efficacy of xylitol-containing and conventional chewing gums in reducing salivary counts of Streptococcus mutans: An in vivo study. J Int Soc Prevent Communit Dent 2015;5, Suppl S2:112-7
|How to cite this URL:|
Haghgoo R, Afshari E, Ghanaat T, Aghazadeh S. Comparing the efficacy of xylitol-containing and conventional chewing gums in reducing salivary counts of Streptococcus mutans: An in vivo study. J Int Soc Prevent Communit Dent [serial online] 2015 [cited 2022 Aug 13];5, Suppl S2:112-7. Available from: https://www.jispcd.org/text.asp?2015/5/8/112/172947
| Introduction|| |
Dental caries is an infectious disease that destroys tooth enamel., Dental caries is a multifactorial disease. It is the cumulative result of consecutive cycles of demineralization and remineralization at the interface between the biofilm and the tooth surface. Upon this acid challenge, the hydroxyapatite crystals are dissolved from the subsurface. A number of etiologic factors, e.g., the presence of cariogenic microorganisms, regular consumption of refined carbohydrates, and poor oral hygiene, contribute to the development of dental caries.,,
Dental caries occurs as a result of imbalance between the remineralization and demineralization processes as the interface between the enamel surface and dental biofilm.,,
Dental caries should be prevented in children., Dental caries is preventable in its early stages. Some favorable properties of sugar (sucrose) are its availability and, most importantly, its cost and public perception, which made it(sucrose) acceptable by the public. Replacing sugar (sucrose) with a suitable substitute to combat dental caries is an option that is wide open. Recent, encouraging studies suggest antimicrobial properties as well as lower cariogenicity of some sugar substitutes such as xylitol. In the beverage field, it is clear that artificial sweeteners formed new products that were additional to the sugar-sweetened products and not competitive with them. Saccharin can substitute for the taste of sugar; lactose or sorbitol for the weight or bulk of sugar; xylitol for both taste and bulk. Similarly, the point remains that the per capita consumption of sugar has been stable for decades, in spite of the use of saccharin, cyclamate, and now aspartame. Xylitol is a polyalcohol known to be capable of reducing dental caries by 50%., As xylitol is almost nonfermentable by plaque bacteria, it can inhibit the proliferation, growth, and accumulation of oral bacteria and reduce dental plaque adhesion. Long-term consumption of xylitol has been associated with reduced growth and activity of Streptococcus mutans and lower numbers of these microorganisms in both dental plaque and saliva. These beneficial effects of xylitol render it a good option for the prevention of dental caries.
Numerous studies have evaluated the effects of xylitol on dental caries.,, However, they have reported contradictory results. While some researchers have confirmed the efficacy of this substance in preventing caries,,,, others have rejected such benefits., Wennerholm et al., Isotupa et al., and Twetman  compared the effects of various xylitol and sorbitol concentrations on S. mutans counts in normal individuals, subjects with fixed braces, and 2–4-year-old children, respectively. We compared the effects of xylitol and glucose-containing chewing gums among adults with poor–moderate oral hygiene, and their findings were similar to ours.
Considering the significance of dental caries prevention and the antibacterial effects of xylitol, the present study sought to compare the effects of chewing xylitol-containing gums and conventional gums on S. mutans in the saliva of subjects with moderate and poor oral hygiene.
| Materials and Methods|| |
In this experimental research, 32 individuals (16 dental students and 16 patients visiting dental clinics) who were aged 18–35 years were recruited. The number of participants was selected based on recent studies.,, The participants' oral hygiene was categorized as moderate or poor based on the caries risk assessment table. Individuals were not included if they had any systemic diseases, history of head and neck radiography, or sensitivity to xylitol. Persons receiving any type of antibiotics were not included either. The study protocol was approved by the Ethics Committee of Shahed University, and the participants signed informed consent forms.
Xylitol chewing gums (Orion Food Vina Co. Ltd, Lai Thieu Townlet, Thuan An District, Vietnam) containing 70% xylitol, isomalt, gum arabic, and calcium lactate were purchased from a gum wholesaler in Tehran, Iran [Figure 1]. The gums were 1.5 g each and were approved by the Iranian Dental Association. PK ® gums (1.6 g, Wrigley, Vadapalani, Chennai, India) containing sucrose, glucose, gum, mint extract, pigments, and titanium dioxide were also purchased. The chewing gums were packed in similar 30-gum packages and distributed among the participants. All subjects were instructed to take three gums a day after main meals (breakfast, lunch, and dinner) and chew each gum for 15 min.
|Figure 1: Xylitol chewing gums (Orion Food Vina Co. Ltd, Lai Thieu Townlet, Thuan An District , Vietnam Vietnam)|
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A crossover design was used in the current research. The two groups with moderate and poor oral hygiene (N = 16) were each divided into two groups of eight to receive either PK ® gums or xylitol gums during the first 10-day period. After a 10-day washout period, each group received the other type of gum for another 10 days. Saliva samples were collected and tested before and after each stage. It should be noted that 2 participants were excluded from the study due to the administration of antibiotics during the second stage.
The samples were cultured using blood agar powders (Ceneda Co., Germany). At each stage, the culture medium was prepared by mixing 40 gr blood agar powder with 1 L distilled water. The mixture was then boiled and the resultant clear solution was autoclaved at a temperature of 121°C and a pressure of 15 atm for 15 min. After cooling the sterilized solution down to 45–50°C, 50 mL defibrinated sheep blood was added to the medium. The result was a blood agar culture medium containing 5% defibrinated sheep blood. The culture medium was finally transferred to 8-cm plates (Farazbin Co., Tehran, Iran) and the plates were maintained at 4°C until use.
Mitis Salivarius-Bacitracin Agar (MSBA), (Ceneda Co., Germany) was applied to differentiate between colonies suspected to be S. mutans and other colonies formed on blood agar plates. In order to prepare MSBA plates, a mixture of 25 medium powder and 250 mL sterile distilled water was boiled to obtain a clear solution. At the same time, 0.25 g potassium tellurite (measured by a sensitive laboratory scale) was mixed with 25 mL sterile distilled water and the mixture was autoclaved (at 121°C and 15 atm) with the medium solution for 15 min. After sterilization, the containers holding the medium and potassium tellurite were cooled down to 45–50°C. The potassium tellurite solution and 0.25 g bacitracin were then added to the medium solution to produce MSBA. The resultant medium was finally moved to 8-cm plates (Farazbin Co., Iran) and kept at 4°C until required for the experiments.
Sampling and microbial culturing were performed at baseline, after the first and second 10-day periods of gum consumption, and after the washout period. At the time of sampling, the participants were asked rinse their mouths with water immediately after getting up in the morning (before eating breakfast or brushing their teeth). Afterward, 0.5 mL samples of unstimulated saliva were collected in sterile capped tubes (Greiner Bio-One, Frickenhausen, Germany) and immediately transferred to the microbiology laboratory of Imam Ali Clinic (Tehran, Iran).
In the laboratory, an inoculating loop was flamed and sterilized. Then, saliva samples were transferred to the blood agar medium using the loop [dilution factor: 102 colony-forming units (cfu)/mL]. After placing the plates in a candle jar (containing 5% carbon dioxide), the media were incubated at 37°C. After 24 h, colonies suspected to be S. mutans were placed on Petri dish More Detailses and Gram staining was performed to confirm the presence of S. mutans. A sterile loop was used to move the grown colonies of Streptococcus from the blood agar medium to the MSBA. This process was performed near a flame. The MSBA was then placed in a candle jar and incubated at 37°C for 24 h. Colonies of S. mutans (dark blue colonies 2–3 mm in size) were then observed on the culture medium. When the presence of S. mutans was confirmed, the number of S. mutans colonies formed on the blood agar medium was counted. The number of CFUs was identified by morphology, counted in a stereomicroscope, and expressed as CFU mL -1. Ultimately, the collected data were analyzed with paired t-tests. All statistical analyses were conducted using SPSS for Windows (SPSS Inc., Chicago, IL, USA).
| Results|| |
A total of 32 individuals aged 18–35 years were allocated to two groups of 16 based on their oral hygiene status (poor or moderate). The study consisted of two stages. Saliva samples were collected and tested before and after each stage. In the group with moderate oral hygiene, the colony count at baseline ranged between 4 × 102 CFU/mL and 9 × 102 CFU/mL. These values were reduced to 2–6 × 102 CFU/mL after the first stage of the study. Further reductions in the mean number of colonies were observed after the second stage (7 × 102 CFU/mL at baseline versus 4 × 102 CFU/mL after the second stage).
In the group with poor oral hygiene, colony counts at baseline (22–50 × 102 CFU/mL) were reduced after the first stage of the study (12–38 × 102 CFU/mL). The mean value measured after the second stage was also lower than the baseline value (19 × 102 CFU/mL vs 27 × 102 CFU/mL).
Overall, 16 subjects with poor and moderate oral hygiene used xylitol gums. The mean baseline colony count in this group (19 × 102 CFU/mL) declined after xylitol chewing gums three times a day for 10 days (11 × 102 CFU/mL). The mean number of colonies was also considerably lower after the second 10 days of xylitol chewing gums than after the washout period (10 × 102 CFU/mL vs 17 × 102 CFU/mL).
The other 16 people with poor and moderate oral hygiene used PK ® gums. The mean colony count in this group was 18 × 102 CFU/mL, which was lowered to 15 × 102 CFU/mL following 10 days of chewing PK ® gums three times a day. The value after the washout period (16 × 102 CFU/mL) was also reduced to 13 × 102 CFU/mL after another 10 days of PK ® chewing gums. As the mean reduction was higher in the xylitol group than in the PK ® group (7.5 × 102 CFU/mL vs 3 × 102 CFU/mL), the xylitol-containing gum was more effective.
In the group with moderate oral hygiene, paired t-test revealed a significant difference in the mean count of S. mutans colonies before and after xylitol gum consumption (P = 0.000002). The same test also suggested a significant difference between the numbers of S. mutans colonies after the 10-day washout period and after the second 10 days of chewing gums (P < 0.05).
In the group with poor oral hygiene, significant differences were detected between the mean S. mutans colony counts before and after 10 days of xylitol chewing gums (P < 0.01) and before and after the second stage of chewing gums (P < 0.05).
Similarly, significant differences in the mean colony counts before and after the first stage of PK ® gum consumption were confirmed in subjects with both poor and moderate oral hygiene (P < 0.01). Likewise, the mean colony counts before and after the first stage of PK ® gum consumption were significantly different in participants with both poor and moderate oral hygiene (P < 0.05) [Table 1].
|Table 1: Streptococcus mutans colony counts in different groups and at different time intervals|
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| Discussion|| |
Xylitol, a nonfermentable polyol, is a natural sweetener that does not promote tooth decay., It increases salivary flow, which in turn stimulates the remineralization process and inhibits bacterial growth and metabolism in dental plaques., Despite these properties, the efficacy of xylitol as an anticaries agent is still under debate.,
The present study compared the effects of xylitol-containing and conventional chewing gums on salivary levels of S. mutans. Our results indicated the effectiveness of xylitol in inhibiting S. mutans growth among people with poor–moderate oral hygiene.
S. mutans is the most important microorganism involved in dental caries. The acid produced following the fermentation of sugar and sugar alcohols by S. mutans can destroy tooth enamel and result in dental caries. It, however, cannot ferment xylitol, which contains five carbon atoms. Furthermore, xylitol can inhibit the growth of S. mutans by creating an alkaline environment in the mouth. Xylitol is also able to form a complex with calcium. The formation of such complexes in the mouth will promote the remineralization of tooth enamels that have previously lost their minerals.,
Although Makinen et al. reported similar results, our results are more reliable due to the use of a crossover design. Wennerholm et al., Isotupa et al., and Twetman and Stecksen-Blick  compared the effects of various xylitol and sorbitol concentrations on S. mutans counts in normal individuals, subjects with fixed braces, and 2–4-year-old children, respectively. While we compared the effects of xylitol and glucose-containing chewing gums among adults with poor–moderate oral hygiene, their findings were similar to ours.
In a randomized clinical trial, Ritter evaluated 21–80-year-old individuals in terms of occlusal and smooth surface caries over a 33-month period. The results confirmed the beneficial role of xylitol in preventing root caries in adults with active decay. We, however, adopted a crossover approach and recorded bacterial counts in two different stages. Despite differences in methodology, our study and that of Ritter's yielded similar results. Nevertheless, our findings might be more accurate because we calculated colony counts after the chewing of both types of gums.
In contrast to our findings, Duane followed preschool children for 9 months and 21 months and reported lozenges, xylitol/maltitol, and erythritol/maltitol to be ineffective in reducing dental caries. This difference can be attributed to the type of intervention and the age group of the participants (18–35 years in the present research). In addition, we used a crossover design to determine microbial counts after the chewing of gums.
The frequency of chewing gums, rather than the exact concentration of xylitol, seems to be the important determinant of reduction in salivary levels of S. mutans and tooth decay prevention. In fact, chewing gums should be used at least three times a day to reduce dental caries by 40–50%. We followed the same procedure and observed reductions in salivary levels of S. mutans. Long-term application of xylitol is believed to inhibit the growth and activity of S. mutans and prevent dental caries.
In the current study, chewing gums (either xylitol or PK ®) could more effectively decrease salivary levels of S. mutans in subjects with poor oral hygiene (without usual hygienic measures) than in those with moderate oral hygiene (with usual hygienic measures). In the absence of all usual hygienic measures in the group with poor oral hygiene, chewing gums could dramatically reduce S. mutans counts in their saliva. It is also noteworthy that in both groups, xylitol (which did not contain sucrose) was more effective than PK ® (containing sucrose). This finding can be justified by the intrinsic capabilities of sugar substitutes to decrease salivary levels of S. mutans and control the acidity of saliva.
The present study adopted a crossover design. As all subjects were both cases and controls, our results regarding the effects of the intervention (xylitol or PK ®) were more reliable than other, similar studies. To the best of our knowledge, no previous research has ever used this design. Moreover, previous research has generally used higher frequencies of chewing gums or longer periods of follow-up. Considering the significance of lowering salivary levels of S. mutans in individuals with poor oral hygiene, we compared the effects of xylitol-containing and conventional chewing gums in people with poor and moderate oral hygiene. We observed both types of gums, especially xylitol, to effectively reduce salivary levels of S. mutans in the two groups. This beneficial effect was more noticeable in participants with poor oral hygiene.
The major limitation of the present study was the lack of exact supervision in the appropriate use of chewing gums.
Considering the effects of xylitol in decreasing dental plaques, further studies are recommended to assess the effects of this polyol on periodontal diseases. Researchers are also suggested to evaluate the effects of xylitol-containing mouthwashes, toothpastes, and candies on oral bacteria and periodontal diseases.
| Conclusion|| |
Dental caries is a multifactorial, diet-associated infectious disease. Based on the results of this study, sucrose-free (xylitol) chewing gums when chewed 3 times a day for 15 min over a period of 10 days were more effective than sucrose-containing chewing gums in reducing salivary levels of S. mutans, especially in subjects with poor oral hygiene. Using these chewing gums can thus be recommended as an effective substitute for mechanical methods in individuals with poor oral hygiene or mental and/or physical disabilities.
Financial support and sponsorship
Department of Pediatric Dentistry, Shahed University.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Buzalaf MA. Fluoride and the oral environment. Monogr Oral Sci 2011;22.:97-114.
Fejerskow O, Kidd, EA, Nyvad B, Baclum V. Defining the disease: An introduction. In: Fejerskov O, Kidd E, editors. Dental Caries-The Disease and its Clinical Management. 2nd
ed. Oxford: Blackwell Munksgaard; 2008. P
Selwitz RH, Ismail AI, Pitts NB. Dental caries. Lancet 2007;369:51-9.
Bader JD, Rozier RG, Lohr KN, Frame PS. Physicians' roles in preventing dental caries in preschool children: A summary of the evidence for the U.S. Preventive Services Task Force. Am J Prev Med 2004;26:315-25.
U.S. Department of Health and Human Services. Oral Health in America: A Report of the Surgeon General. Rockville, MD: U.S. Department of Health and Human Services, National Institute of Dental and Craniofacial Research, National Institutes of Health; 2000. p. 17-21.
Caufield PW, Griffen AL. Dental caries: An infectious and transmissible disease. Pediatr Clin North Am 2000;47:1001-19, v.
Tinanoff N, Reisiine S. Update on early childhood caries since the surgeon general's report. Acad Pediatr 2009;9:396-403.
Featherstone JD. The continuum of dental caries-evidence for a dynamic disease process. J Dent Res 2004;83:C39-42.
Dawes C. What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc 2003;69:722-4.
Khoo G, Zhan L, Hoover C, Featherstone JD. Cariogenic virulence characteristics of mutans streptococci isolated from caries-active and caries-free adults. J Calif Dent Assoc 2005;33:973-80.
Casamassimo PS, Thikkurissy S, Edelstein BL, Maiorini E. Beyond the dmft: The human and economic cost of early childhood caries. J Am Dent Assoc 2009;140:650-7.
Kawashita Y, Kitamura M, Saito T. Early childhood caries. Int J Dent 2011;2011:725320.
Chandrashekar BR, Suma S, Sukhabogi JR, Manjunath BC, Kallury A. Oral health promotion among rural school children through teachers: An interventional study. Indian J Public Health 2014;58:235-40.
Roshan N, Sakeenabi B. Practical problems in use of sugar substitutes in preventive dentistry. J Int Soc Prev Community Dent 2011;1:1-8.
Bader J, Shugars D, Bonito A. A systematic review of selected caries prevention and management methods. Community Dent Oral Epidemiol 2001;29:399-411.
Lenkkeri AM, Pienihäkkinen K, Hurme S, Alanen P. The caries-preventive effect of xylitol/maltitol and erythritol/maltitol lozenges: Results of a double-blinded, cluster-randomized clinical trial in an area of natural fluoridation. Int J Paediatr Dent 2012;22:180-90.
Mäkinen KK, Hujoel PP, Bennett CA, Isotupa KP, Mäkinen PL, Allen P. Polyol chewing gums and caries rates in primary dentition: A 24-month cohort study. Caries Res 1996:30:408-17.
Beiswanger BB, Boneta AE, Mau MS, Katz BP, Proskin HM, Stookey GK. The effect of chewing sugar free gum after meals an clinical incidence. J Am Dent Assoc 1998:129:1623-6.
Bader JD, Vollmer WM, Shugars DA, Gilbert GH, Amaechi BT, Brown JP, et al
. Results from the Xylitol for Adult Caries Trial (X-ACT). J Am Dent Assoc 2013;144:21-30.
Scheie AA, Fejerskov OB. Xylitol in caries prevention: What is the evidence for clinical efficacy? Oral Dis 1998;4:268-78.
Twetman S. Current controversies--is there merit? Adv Dent Res 2009;21:48-52.
Mäkinen KK. Xylitol-based caries prevention: Is there enough evidence for the existence of a specific xylitol effect? Oral Dis 1998;4:226-30.
Hayes C. The effect of non-cariogenic sweeteners on the prevention of dental caries: A review of the evidence. J Dent Educ 2001;65:1106-9.
Van Loveren C. Sugar alcohols: What is the evidence for caries-preventive and caries-therapeutic effects? Caries Res 2004;38:286-93.
Deshpande A, Jadad AR. The impact of polyol-containing chewing gums on dental caries: A systematic review of original randomized controlled trials and observational studies. J Am Dent Assoc 2008;139:1602-14.
Mäkinen KK. Sugar alcohols, caries incidence, and remineralization of caries lesions: A literature review. Int J Dent 2010;2010:981072.
Antonio AG, Pierro VS, Maia LC. Caries preventive effects of xylitol-based candies and lozenges: A systematic review. J Public Health Dent 2011;71:117-24.
Wennerholm K, Arends J, Birkhed D, Ruben J, Emilson CG, Dijkman AG. Effect of xylitol and sorbitol in chewing gums on mutans strptococci.plaque PH and mineral loss of enamel. Caries Res 1994;28:48-54.
Isotupa KP, Gunn S, Chen CY, Lopatin D, Mäkinen KK. Effect of polyol gums on dental plaque in orthodontic patients. Am J Orthod Dentofacial Orthop 1995;107:497-504.
Twetman S, Stecksen-Blicks C. Effect of xylitol-containing chewing gums on lactic acid production in dental plaque form caries active pre-school children. Oral Health Prev Dent 2003;1:195-9.
Ghasempour M, Sefdgar SA, Moghadamnia AA, Ghadimi R, Gharekhani S, Shirkhani L. Comparative study of Kefir yogurt-drink and sodium fluoride mouth rinse on salivary mutans streptococci. J Contemp Dent Pract 2014;15:214-7.
Emamieh S, Khaterizadeh Y, Goudarzi H, Ghasemi A, Baghban AA, Torabzadeh H. The effect of two types chewing gum containing casein phosphopeptide-amorphous calcium phosphate and xylitol on salivary Streptococcus mutans. J Conserv Dent 2015;18:192-5.
Lakade LS, Shah P, Shirol D. Comparison of antimicrobial efficacy of chlorhexidine and combination mouth rinse in reducing the Mutansstreptococcus count in plaque. J Indian Soc Pedod Prev Dent 2014;32:91-6.
Maguire A, Rugg-Gunn AJ. Xylitol and caries prevention-is it a magic bullet? Br Dent J 2003;194:429-36.
Milgrom P, Ly KA, Roberts MC, Rothen M, Mueller G, Yamaguchi DK. Mutans streptococci dose response to xylitol chewing gum. J Dent Res 2006;85:177-81.
Ly KA, Milgrom P, Roberts MC, Yamaguchi DK, Rothen M, Mueller G. Linear response of mutans streptococci to increasing frequency of xylitol chewing gum use: A randomized controlled trial [ISRCTN43479664]. BMC Oral Health 2006;6:6.
Söderling EM. Xylitol, mutans streptococci, and dental plaque. Adv Dent Res 2009;21:74-8.
Mäkinen KK, Pemberton D, Mäkinen PL, Chen CY, Cole J, Hujoel PP, et al
. Polyol-combinant saliva stimulants and oral health in Veterans Affairs patients-an exploratory study. Spec Care Dentist 1996;16:104-15.
Aizawa S, Miyasawa-Hori H, Nakajo K, Washio J, Mayanagi H, Fukumoto S, et al
. Effects of alpha-amylase and its inhibitors on acid production from cooked starch by oral streptococci. Caries Res 2009;43:17-24.
Sano H, Nakashima S, Songpaisan Y, Phantumvanit P. Effect of a xylitol and fluoride containing toothpaste on the remineralization of human enamel in vitro
. J Oral Sci 2007;49:67-73.
Mäkinen KK, Alanen P, Isokangas P, Isotupa K, Söderling E, Mäkinen PL, et al
. Thirty-nine-month xylitol chewing-gum programe in initially 8-year old school children: A feasibility study focusing on mutans streptoco cci and lactobacilli. Int Dent J 2008;58:41-50.
Duane BG. No evidence of caries reduction found in a school xylitol and erythritol lozenge programme. Evid Based Dent 2011;12:102-3.
Mortazavi M. Role of xylitol in dental caries prevention. J Dentistry 2002;20:360-9.
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