Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions| Reviewers

  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 177    
Year : 2021  |  Volume : 11  |  Issue : 5  |  Page : 525-530

Fungicidal effect of lemongrass essential oil on Candida albicans biofilm pre-established on maxillofacial silicone specimens

1 Private Practice, Kuala Lumpur, Malaysia
2 Maxillofacial Prosthodontics, Department of Prosthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
3 Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
4 Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Correspondence Address:
Asst. Prof. Suwan Choonharuangdej
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok.
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jispcd.JISPCD_63_21

Rights and Permissions

Aims: This in-vitro study aimed to evaluate the efficacy of lemongrass (Cymbopogon citratus) essential oil in eradicating Candida albicans biofilm pre-established on the maxillofacial silicone specimens. Materials and Methods: Two maxillofacial silicones, namely, MDX4-4210 and Multisil Epithetik, were used for the fabrication of 6 mm diameter disks (n = 21 for each brand of silicone). A 48-h mature C. albicans ATCC 10231 biofilm was pre-established on sterile silicone specimen. These disks were then exposed to various concentrations of lemongrass essential oil ranging from 0.31% to 5% (v/v), 20% (v/v) nystatin, and RPMI-1640 medium for 18–20 h. After exposure, the remaining viable fungal biofilm was examined by the XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide]-reduction assay. All data were analyzed by using a regression coefficient and a post hoc Tukey HSD multiple comparisons test (α = 0.05). Results: Different brands of silicone used for fabrication did not significantly affect the formation of mature C. albicans biofilm (P =0.302). A 5% (v/v) lemongrass essential oil significantly eliminated fungal biofilm by approximately 95% (P =0.031). However, less than 50% of the fungal biofilm was eliminated by the tested oil at a concentration as low as 0.31% (v/v). Furthermore, the fungicidal efficacy against C. albicans biofilm of lemongrass essential oil at 2.5% (v/v) was as potent as that of 20% (v/v) nystatin suspension (P = 0.99). Conclusion: Lemongrass essential oil expressed fungicidal effect on C. albicans biofilm pre-established on the disks fabricated from different brands of silicone. Additionally, the fungicidal effectiveness of the oil against the mature fungal biofilm was dose-dependent.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded95    
    Comments [Add]    
    Cited by others 1    

Recommend this journal