Journal of International Society of Preventive and Community Dentistry

REVIEW ARTICLE
Year
: 2016  |  Volume : 6  |  Issue : 6  |  Page : 509--516

In vitro and ex vivo microbial leakage assessment in endodontics: A literature review


Sohrab Tour Savadkouhi, Hengameh Bakhtiar, Safoura Emami Ardestani 
 Department of Endodontic, Dental Branch of Islamic Azad University, Tehran, Iran

Correspondence Address:
Sohrab Tour Savadkouhi
10th Neyestan, Pasdaran Ave, Tehran
Iran

The aim of this study was to perform a literature review of published in-vitro and ex-vivo studies, which evaluated microbial leakage in endodontics in the past 10 years. A comprehensive electronic literature search was carried out in PubMed database for English articles published from 2005 to 2016 using the keywords “endodontics,” “in vitro,” “ex vivo,” “microbial leakage,” “microbial penetration,” “saliva,” “Enterococcus faecalis,” “E. faecalis,” “endodontic sealers,” “temporary filling material,” “apical plug,” “mineral trioxide aggregate,” and “MTA.” The keywords were combined using Boolean operators AND/OR. Based on our search strategy, 33 relevant articles were included in the study. There are three main methods for assessment of bacterial microleakage, namely, (A) the dual-chamber leakage model, (B) detection of bacteria using a scanning electron microscope (SEM), and (C) polymerase chain reaction. All bacterial leakage models have some limitations and may yield different results compared to other microleakage evaluation techniques (i.e., dye penetration, fluid filtration, or electrochemical tests). The results of SEM correlated with those of microbial leakage test in most studies. Microbial leakage test using saliva better simulates the clinical setting for assessment of the leakage of single or mixed bacterial species.


How to cite this article:
Savadkouhi ST, Bakhtiar H, Ardestani SE. In vitro and ex vivo microbial leakage assessment in endodontics: A literature review.J Int Soc Prevent Communit Dent 2016;6:509-516


How to cite this URL:
Savadkouhi ST, Bakhtiar H, Ardestani SE. In vitro and ex vivo microbial leakage assessment in endodontics: A literature review. J Int Soc Prevent Communit Dent [serial online] 2016 [cited 2022 May 27 ];6:509-516
Available from: https://www.jispcd.org/article.asp?issn=2231-0762;year=2016;volume=6;issue=6;spage=509;epage=516;aulast=Savadkouhi;type=0